中国科学家发现新种芽孢杆菌:兵马俑芽胞杆菌(Bacillus bingmayongensis sp. nov.)

摘 要:

中国从西安秦始皇兵马俑1号坑土壤中分离到一个芽孢杆菌样菌株新种:兵马俑芽胞杆菌(Bacillus bingmayongensis sp. nov.)。16S rRNA序列比较分析证实该分离株属于芽孢杆菌属,针对gyrB基因序列进行的系统进化分析表明FJAT-13831(T)分离株属于一个独立的新种(species)。

关键词:

中国和德国科学家共同于国际权威的Antonie van Leeuwenhoek杂志2014年第3期发表文章(参考文献:Liu B, Liu GH, Hu GP, Cetin S, Lin NQ, Tang JY, Tang WQ, Lin YZ. Bacillus bingmayongensis sp. nov., isolated from the pit soil of Emperor Qin's Terra-cotta warriors in China. Antonie Van Leeuwenhoek, 2014,105(3):501-510.),从西安秦始皇兵马俑1号坑土壤中分离到一个芽孢杆菌样菌株FJAT-13831(T),该菌株的主要脂肪酸包括iso-C15:0、iso-C17:0、C16:0、iso-C13:0、anteiso-C15:0和iso-C17:1ω5c。16S rRNA序列比较分析证实该分离株属于芽孢杆菌属,与假蕈状芽孢杆菌(Bacillus pseudomycoides)DSM 12442(T)株的同源性最高(99.72 %)。针对gyrB基因序列进行的系统进化分析表明FJAT-13831(T)分离株属于一个独立的种,与DSM 12442(T)菌株的相似性为93.8 % (<95 %)。全基因组系统进化分析表明FJAT-13831(T)分离株与DSM 12442(T)菌株的同源性为91.47 (<95 %),这些研究表明FJAT-13831(T)分离株属于芽孢杆菌的一个新种(species)。

原文摘要如下:

Abstract: A Bacillus-like isolate, strain FJAT-13831(T), isolated from the No. 1 pit soil of Emperor Qin's Terra-cotta Warriors in Xi'an City, China, was studied to determine its taxonomic status. Dominant fatty acids of this organism included iso-C15:0, iso-C17:0, C16:0, iso-C13:0, anteiso-C15:0, and iso-C17:1ω5c. Comparative 16S rRNA gene sequence analysis confirmed the affiliation of this isolate to the genus Bacillus and indicated that it was closely related to Bacillus pseudomycoides DSM 12442(T) (99.72 % similarity). A phylogenetic analysis of the gyrB gene sequence similarities exhibited independent clustering of the isolate FJAT-13831(T) and showed 93.8 % (<95 %) sequence similarity with its closest phylogenetic neighbour B. pseudomycoides DSM 12442(T). Separate standing of the strain FJAT-13831(T) was supported by a whole genome-based phylogenetic analysis with an average nucleotide identity value of 91.47 (<95 %) between isolate FJAT-13831(T) and B. pseudomycoides DSM 12442(T) and was consistent with the results of DNA-DNA hybridization (69.1 % relatedness). These findings support the conclusion that the isolate FJAT-13831(T) represents a novel species, for which the name Bacillus bingmayongensis sp. nov. is proposed. The type strain is FJAT-13831(T) (= CGMCC 1.12043(T) = DSM 25427(T)).

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目前评论:1   其中:访客  1   博主  0

  1. avatar Emily

    准确的说,应该命名为:鞋底芽孢杆菌。
    因为后来证实:原始样品并不是来自于西安秦始皇兵马俑1号坑,而是采样人员在秦始皇兵马俑旅游后,从鞋底上刮下的土壤中分离到该菌株,因此个人认为更准确的名称应该是鞋底芽孢杆菌。

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